Principle Areas of Study
Define the role of carbohydrate binding proteins in immunity
In addition to studying factors the directly impact the growth and survival of microbes that decorate themselves in blood group antigens, we are also seeking to understand fundamental processes whereby carbohydrate-binding proteins regulate immune function. These studies include factors regulating alterations in cell surface glycosylation of key immune effector cells, and the impact of these changes on the binding and subsequent signaling of different immune populations. In addition, these studies include the development and utilization of various glycan microarray platforms to examine the binding specificity of various carbohydrate binding proteins.
As various carbohydrate binding proteins possess the capacity to significantly impact the development and regulation of immunity, positively manipulating immunity through altering interactions of immune cells with various carbohydrate binding proteins can provide a unique strategy to directly alter immune function. Such strategies may be used to specifically inhibit immunity against various alloantigens in the setting of autoimmunity, transfusion and transplantation or to augment immunity during infectious disease or just prior to vaccination. As a result, these studies provide the opportunity to significantly and positively impact the role of immunity in a variety of ailments, ranging from neoplasia to autoimmunity and infectious disease.
Lab members working in this area
Connie Arthur, Jianmei Wang
- Stowell SR, Qian Y, Karmakar S, Koyama NS, Dias-Baruffi M, Leffler H, McEver RP, Cummings RD. Differential roles of galectin-1 and galectin-3 in regulating leukocyte viability and cytokine secretion. Journal of Immunology 2008 Mar; 180(5): 3091-3102.
- Stowell SR, Karmakar S, Stowell CJ, Dias-Baruffi M, McEver RP, Cummings RD. Human galectin-1, -2, and -4 induce surface exposure of phosphatidylserine in activated human neutrophils but not in activated T cells. Blood 2007 Jan; 109(1): 219-227.
- Arthur CM, Baruffi MD, Cummings RD, Stowell SR. Evolving Mechanistic Insights into Galectin Functions. Methods in Molecular Biology 2015;1207:1-35.
- Arthur CM, Cummings RD, Stowell SR. Using Glycan Microarrays to Understand Immunity. Current Opinion in Chemical Biology 2014 Feb;18:55-61.